Orbitrap Mass Spectrometry System

Orbitrap Mass Spectrometry System (Orbitrap Exploris 240)

High-resolution mass spectrometry using the Orbitrap Exploris 240 is a state-of-the-art analytical technique for the precise identification, quantification, and isotopic analysis of complex organic compounds. The Orbitrap mass analyser enables high-resolution, accurate-mass (HRAM) detection, providing detailed molecular fingerprints of target analytes. Samples are ionised via electrospray ionisation (ESI) or atmospheric pressure chemical ionisation (APCI), generating charged species that enter the mass analyser. The Orbitrap technology ensures ultra-high mass accuracy and low detection limits, making it ideal for the analysis of trace contaminants, environmental pollutants, and biomolecules.

Beyond conventional mass spectrometry, the Orbitrap Exploris 240 is also used for stable isotope analysis (CSIA) and intramolecular and position-specific stable isotope analysis (PSIA), enabling the detailed tracking of micropollutant sources and transformation pathways in environmental systems. This approach provides critical insights into biodegradation, photodegradation, and other transformation processes, supporting studies in environmental chemistry, forensic science, and biogeochemistry.

The laboratory is equipped with a Thermo Scientific Orbitrap Exploris 240, a high-resolution mass spectrometer designed for targeted and untargeted analysis of organic compounds, including stable isotope ratio measurements. Its advanced spectral resolution and fast scanning capabilities allow for the detection of a wide range of compounds with high specificity.

Orbitrap Exploris 240 Analytical Characteristics

  • Resolution:Up to 240,000 FWHM at m/z 200
  • Mass accuracy: <1 ppm with internal calibration
  • Detection limits:Low nanogram to picogram range
  • Ionisation modes:ESI (positive and negative)
  • Targeted and untargeted screeningofenvironmental contaminants, pharmaceuticals, and metabolites
  • Stable isotope analysis (SIA) and intramolecular stable isotope analysis (ISI) for tracking sources and transformation of micropollutants
  • Calibration using internal and external mass standards